Iiseli zeSistimu kunye neNkcubeko yeZicubu kwi-microbiology

I-YongYyeu igxile kwezovavanyo zenkcubeko yeselula. Njengesixhobo esiqhelekileyo nesibalulekileyo seseli ezikhundleni, iipleyiti zenkcubeko zineemilo ezahlukeneyo, iinkcukacha kunye nokusetyenziswa. Ukukhetha iiplate zenkcubeko: 1. Iipleyiti zenkcubeko zinokwahlulwa zibe ziipleyiti ezisezantsi zeflethi kunye neepleyiti ezijikelezileyo (i-U-Deed kunye ne-V-imile) ngokwemilo yezantsi; 2. Inani leziqula zenkcubeko liyi-6, 12, 48, 96, 384, 1536 Well njalo njalo; Abathengi banokukhetha iiplate zabo zenkcubeko ngokweemfuno zabo zovavanyo. Ewe kunjalo, sikwanazo izitya ze-petri, iiflegi zenkcubeko, imibhobho ye-pasteur, tyhala iintsizi, njl njl. Ukuqhutywa kweemvavanyo zenkcubeko. Ukuba ufuna inkonzo eyenziwe ngokwezifiso, nceda uqhagamshelane neSebe leSiseko sentengiso.

Sahlula kwaye sikhethe phakathi kwenkcubeko eneflethi esezantsi kunye ne-Runder ezantsi (i-U-Deed kunye ne-V-imile)

I-1) Iipleyiti zenkcubeko ezinamazantsi eflethi zisetyenziselwa iiseli ezinamathela.

2) Uhlobo lwe-V lusetyenziselwa ukunqunyanyiswa kwenkcubeko.

I-3) Iipleyiti zenkcubeko ze-U-I-U-I-U-U-I-U-I-USETYENZISIWEYO KUPHELA NGOKUSETYENZISELWE Inkcubeko yeseli.

I-4) Iipleyiti zenkcubeko ezizenziwe ngamanye amaxesha zisetyenziselwa ukuvavanywa kwe-hemaghlolution.

Iinkcukacha ezithe kratya

Imifanekiso engakumbi

Umbuzo: Ndibona ukuba iplate yesiko ineenkcazo ezininzi, ezizezisi-4, 6, 12, ezingama-4 48, 48 ne-48 nama-98 amaqula, kodwa andazi ukuba yeyiphi na into enokuyisebenzisa, nceda ucebise.

Impendulo: Kuxhomekeka kwiimfuno zakho zovavanyo oluthile, ukuhamba nge-cytometry kuhlala kusetyenziswa iplate ye-6, i-MTT isebenzisa iplate yenkcubeko engama-96, inkcubeko yeseli isebenzisa iplate ye-24 yeselfowuni, njl. Kuxhomekeka kuvavanyo lwakho!

Umbuzo: Nguwuphi umahluko phakathi kweplate ye-microtiite kunye neplate yenkcubeko efanelekileyo?

Impendulo: Iipleyiti ze-ELISA zibiza kakhulu kuneziqwenga zenkcubeko. Iiplate zenkcubeko ikakhulu zisetyenziselwa inkcubeko, kodwa inokusetyenziselwa ukulinganisa uxinzelelo lweprotein; Iipleyiti ze-ELISA zibandakanya iipleyiti zokudibanisa kunye neeplate zokuphendula, ezinokusetyenziselwa inkcubeko yeselula. Zisetyenziselwa ikakhulu iNkqutyana yeseli.Protein emva kokuthathwa kweSerunnoenozyme Chain Readetion ifuna iimfuno eziphezulu kunye ne-enzyme ethile eneelebheli.