Iipleyiti ze-Elisa i-Esay Kit

Yongeza Ulawulo: Yongeza ii-3 Wells zolawulo olubi, 1 kakuhle lolawulo oluqinisekileyo, kunye ne-100 ye-serum yokulawula.
I-Buxite: Impendulo ye-Elisa 96 i-EIFA 96 I-Plate ifuna ukungcungcuthekiswa ukwenza isampulu exubeneyo, emva koko ibekwe kwi-37 ° C I-Cubator ye-37 ° lemizuzu engama-20 yokuphendula kangangemizuzu engama-20 yokuphendula.
Hlamba iplate ye-ELISA: Dlula isisombululo sokuhlamba i-15m ngamanzi anemikhono kumthamo we-300ml. Xa ukuhlamba izandla, iziqulatho kumaqula eplate yokuphendula kufuneka ithululelwe, isisombululo sokuhlamba kufuneka sigcwaliswe sisikhundla sendlela yokuphendula, emva koko sibekwe ngamandla. Phinda le nto amaxesha ama-5 kwaye emva koko uqale ukuwome. Ukuhlamba umatshini kufuneka kwenziwe izihlandlo ezi-5, umngxunya ngamnye ukhunjulwe nge-200u ngesisombululo sokuhlamba okanye unokugcwaliswa, emva koko uyeke
Emva kwemizuzu engama-30, drain kunye ne-pat yomile.
Yongeza isisombululo sokuSebenza se-Enzyme: Yongeza i-100 ye-Enzyme ilebhelishana kwi-iquator nganye, yibeke kwi-incubator nge-37, kwaye ihlambe izihlandlo ezi-5 emva kwemizuzu engama-20 yokuphendula. Ukusebenza kokuhlamba i-ELISA MACKT kuyafana nala manyathelo angentla.
Eli linyathelo lokuqala lokuphendula, oluluphuculo lwemibala kunye nokupheliswa kwendlela yokuphendula: I-50 le-50 le-50 yongezwa kwi-ratication kwimpendulo, kwaye umbala uphuhliswa kangangemizuzu eli-10 kubumnyama phantsi kweemeko ezingama-10. Yongeza isisombululo se-Stop kwi-qull nganye
I-50ull yaxubekile ukubantela impendulo.
Ukusetyenziswa kweepleyiti ze-birtiter kufuneka kwenziwe phantsi kweemeko ezingenamgangatho. Ukuzibulala kunye nokucoca kufuneka kwenziwe ngononophelo emva kokusebenzisa iplate ye-microtiita, kodwa kuya kufuneka kuqatshelwe ukuba ezinye ii-enzymes
Phambi kokuthathwa kwebhodi eqhelekileyo, kufuneka ufunde imiyalelo ngononophelo ukuze ubone ukuba inokutshintshwa kubushushu obuphezulu.