Ipleyiti ye-PCR eyenziwe yi-yongyue yezonyango zihlala zahlulwe zangaphezulu kweepleyiti ze-PCR, i-Semi ikhwele ipleyiti ye-PCR kunye neepleyiti ezigcweleyo. Iipleyiti ngaphandle kwesiketi: ihambelana nomjikelo we-thermal okanye oomatshini be-qpcr, kodwa azifanelekanga kwizicelo ezenzekelayo. Uzinzo aluphakami xa upeyiphu, kwaye kufuneka zisetyenziswe ngeplate. I-Semi Skirt Pleit Pleating: Inokutshintshwa ngokufaka ilebheli okanye i-Bacode Isicelo, ilungele isicelo esizenzekelayo kwaye sinozinzo oluhle lokuqhekeza. Isitya esipheleleyo se-PCR PLACR: Kufanelekile kakhulu izicelo zeLebhu ezenzekelayo, kodwa zilungele iilebheli kunye nezicelo zebhakhowudi. Amandla omatshini olungileyo, alungele izixhobo ze-PCR ezinemodyuli zokuphuma, kunye nozinzo oluphezulu ngexesha lemibhobho. Kwi-96 kakuhle ipleyiti ye-PCR okanye i-384 kakuhle iplate ye-PCR, iikobhatha kunye neelebheli ezisekwe kwimisebenzi yeekona kunye neelebheli. Ukusika iikona: Ukukhetha isikhundla sekona esisikiweyo seplate ye-PCR kuxhomekeke kwiimfuno zesixhobo esitshatileyo, esinokuthi sibekwe kakuhle.

Ukuchonga: Ilebheli ye-alphanumeric yeplate ze-PCR inceda ekuchongeni iqula ngalinye kunye neendawo zesampulu. Oku kuhlala kungumbala ophakanyisiweyo wemibala ephakanyisiweyo Kwezinye iimvavanyo zokusetyenziswa kwezicelo ezizenzekelayo, iplate yokuphendula ityhuwe itywiniwe.

I-①wints i-PCR ASSTUSTALS PARD ASTED PP?

Kuba i-PCR / QPROS ENTUTUMSS inonxibelelwano oluqhelekayo nonxibelelwano ngqo neerejista okanye iisampulu ze-billpropylene (i-PP) yindawo ye-biology imbelele kwi-biololenes ezingenamkhenkce kwaye inesifo sokunganyamezeli i-121 ° C kwaye ikwanani nokuguquguquka kobushushu ngexesha lokushiyeka kweqondo lokushisa).

Ukuba ivolumu yesampulu incinci, ityhubhu enye okanye i-tube ye-tube ayikhethileyo. Nangona kunjalo, xa ivolumu enkulu yokuphendula iyafuneka, ityhubhu enye iphambili, kwaye umthamo unokufikelela kwi-0.5 ml. Isiciko seflethi kunye nesiciko sengqungquthela sinezibonelelo zabo. Nceda ujonge le ngcaciso ilandelayo.

Ityhubhu enye: 0.2ml okanye i-0.5ml, ukukhetha isampulu yesampulu.0.2 ml pcr tubes.0.5 ml pups.

I-PCR Tubis: 0.2ml okanye i-0.15ml, 8 Strip Pubr Tubes okanye iityhubhu ezili-12

I-Flat Cap: ibonelela ngosulelo oluchanekileyo lwe-fluorescent kwi-QPCR kwaye iququzelele ukubhala nokufaka iilebheli

I-Convex ikhava: inxibelelana nekhava ye-thermal yesixhobo se-PCR sokunciphisa ukungcungcutheka kwetyhubhu yokuphendula phantsi koxinzelelo; Kodwa ichaphazela ukuhambisa imiqondiso ye-fluorescent kwaye ayinakusetyenziswa kwiimvavanyo ze-QPCR.

② Kutheni le nto ezinye iipleyiti ze-PCR ze-quering ziphuma kwaye ezinye zifihlakele? Yeyiphi engcono?

Lowo ulunge kakhulu. Zimbini iintlobo zepleyiti ze-PCR eziqhelekileyo: iflethi kakuhle kwaye iphakamise imiphetho entle. Iipleyiti ezineflethi kakuhle imiphetho ifanelekile i-thermations. Ukuphendula kweplate kwi-Teals kwi-ofisi kakuhle yokutywina ngokulula, ukunciphisa umngcipheko wokungcoliseka komnqamlezo phakathi kweesampulu.

③ Ukhetha njani ii-PUBR ze-Pubs / iipleyiti ezinamandla ahlukeneyo?

Injongo yokukhetha: Ukukhetha imveliso efanelekileyo ngokweemfuno ezithile zovavanyo. Umthamo we-puby uninzi lwe-PURR lunokuhlangabezana neemfuno zokuphendula kwePRR. Ngokusekwe koku, kuyacetyiswa ukuba isebenzise iityhubhu ezincinci kuqala. Ukuphuculwa kokuphumelela kwe-thermal kwaye kunciphise ukwanda ngenxa yesandi esincinci se-tubector / iipleyiti. Kwangelo xesha, ukhathalelo kufuneka luthathelwe ukunqanda ukongeza kakhulu okanye kuncinci kakhulu xa kongeza iisampulu. Kakhulu unokukhokelela ekuphulukaneni nokuphulukana nenkcitho, ukuchitheka kunye nokungcoliseka komnqamlezo, ngelixa kuncinci kunokubangela ukuthobela isampula ngokulahleka kwengxokolo.

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