Ukukhetha kunye nokusetyenziswa kweeplate zenkcubeko

Njengesixhobo esibalulekileyo esisetyenziswayo kwinkcubeko yeselfowuni, iipleyiti zenkcubeko zinemilo eyahlukeneyo, imigaqo kunye nokusetyenziswa.

Ngaba udidekile ngokukhetha iplate yenkcubeko efanelekileyo?

Andazi ukuba ungayisebenzisa njani iplate yenkcubeko ngokufanelekileyo nangokuchanekileyo?

Ngaba udidekile malunga nendlela yokusebenzisa iBhodi yoQeqesho?

Emva koko, lixesha lokuba baziqhelanise ne-App yokuFunda.

Ukuhlelwa kweeplate zenkcubeko

I-1) Iipleyiti zenkcubeko zinokwahlulwa zibe ngamazantsi eflethi kwaye zijikeleze ezantsi (i-U-Deed kunye ne-V-imile) ngokwemilo yezantsi;

2) Inani leziqula zenkcubeko liyi-6, 12, 48, 96, 384, 1536 Well, njl.;

3) Kuxhomekeka kwizixhobo, kukho iiplate zeTerasaki kunye neeplate eziqhelekileyo zenkcubeko. Ukukhetha kuxhomekeke kuhlobo lweeseli ezikhutshelweyo, ivolumu yenkcubeko efunekayo, kunye nenjongo yovavanyo olwahlukileyo.

1. Umahluko kunye nokukhethwa kwezantsi kweflethi kunye ne-Rive ezantsi (i-U-Deed kunye ne-V-Maled) yeNkcubeko.

Iiplate zenkcubeko esezantsi zisetyenziselwa iiseli ezinamathela;

Chwetheza v isetyenziselwa ukunqunyanyiswa kwenkcubeko;

Iiplate zenkcubeko ye-U-Deper nazo zisetyenziselwa ukulima iiseli zokumiswa;

Chwetheza iipleyiti ze-v ngamanye amaxesha zisetyenziswa kwiimvavanyo ze-hemaglutication.

Iimilo ezahlukeneyo zeipleyiti zenkcubeko zinceda iinjongo ezahlukeneyo. Iiseli ezifundwayo zihlala zinezantsi, ezilungele ukuqaphela kwe-microscope, indawo esezantsi icacile, kwaye ukuphakama kwenkcubeko ye-selt isesikweni, nto leyo efanelekileyo yokufunyanwa kwe-MTT. Ke ngoko, nokuba ziiseli ezihlelelekileyo okanye iiseli zokumiswa, iimvavanyo ezinje nge-MTT isebenzisa iipleyiti ezisezantsi. I-Absorben kufuneka ilinganiswe ukuba isebenzise iiplate zenkcubeko.

Iipleyiti ze-U-Deped okanye ze-V-valed zihlala zisetyenziselwa iimfuno ezithile ezizodwa. Umzekelo, ekumfanekisweni, xa ii-lymphocytes ezimbini ezixutywe kwinkcubeko, kufuneka zinxibelelane nomnye ukuze zikhuthaze. Okwangoku, iipleyiti ezenziwe nge-U-US zisetyenziselwa ukusetyenziswa kuba iiseli zidityaniswe kwindawo encinci.

Iipleyiti zenkcubeko ezijikelezileyo zisetyenziselwa iimvavanyo zokufaka i-isotope ezifuna ukuvuna iiseli ezinomvuni weseli, umz. B. "Inkcubeko ye-Lymphoyte iNqaku", njl.njl. Ukubulala iiseli zokubulala iiseli kungatshintshwa yipleyiti ye-U-I-U-Deed (isantya esisezantsi kwentsimbi emva kongeza iiseli).

Uninzi lweenkcubeko zeseli zisebenzisa iipleyiti zenkcubeko ezihlangeneyo, ezilungele ukuqaphela kwe-microscope, indawo esezantsi icacile, kwaye inqanaba le-Lisellity ye-iCley isebenza rhoqo, elilungele ukusetyenziswa kwe-MTT.

2. Umahluko phakathi kweplate yenkcubeko kunye neplate ye-microtiiter

Iipleyiti ze-Elisa zihlala zibiza kakhulu kwiiplate zenkcubeko. Iipleyiti zeseli ikakhulu zisetyenziselwa ikakhulu kwinkcubeko yeseli, kwaye inokusetyenziselwa ukumisela uxinzelelo lweprotein; Iipleyiti ze-Elisa zibandakanya iiplate ezihlanganisiweyo kunye neeplate zokuphendula, kwaye azisetyenziselwa inkcubeko yeselfowuni. Zisetyenziswa ikakhulu kwimithetho enxulumene ne-ugononzyme. Ukufunyanwa kweProtein yokugqibela kufuna iimfuno eziphezulu kunye ne-enzyme ethile efakwayo.

3. Indawo yequla lequla leepleyiti ezahlukeneyo ezisetyenziswa rhoqo zenkcubeko kunye nenani elicetyiswayo lolwelo ukuba longezwe

Inqanaba lolwelo lwenkcubeko eyongezwe kwiipleyiti ezahlukeneyo ze-ortifice akufuneki ukuba zinzulu kakhulu, ngokubanzi mm, kunye nexabiso elifanelekileyo lolwelo olunokuthi lubalwe ngokusekwe kwindawo ye ezantsi kweplate ye-orifice. Intwasahlobo eyahlukileyo. Ukuba isixa sexabiso elongeziweyo sininzi kakhulu, iya kuchaphazela ukutshintshiselana ngegesi (ioksijini), kwaye kulula ukuphuphuma kwaye kubangele ukungcola ngexesha lokuhamba. Ukuxinana okukhethekileyo kwesesi kunokuba kulawulwa ngokuguquguquka ngokwenjongo yovavanyo. I-YongYyei ye-Yongyie yezolimo yeselfowuni yenziwa nge-polystyrene yonyango (i-PS), iveliswe kwindibano yokucoca i-100,000 ye-100,000, ifakwe i-Irradiation, ayinayo i-dnase, i-RNAK, ikhuselekile kwaye ikhuselekile kwaye ikhuselekile kwaye ikhuselekile kwaye ikhuselekile kwaye ikhuselekile kwaye ikhuselekile kwaye ikhuselekile kwaye ikhuselekile kwaye ikhuselekile kwaye ikhuselekile kwaye ikhuselekile. I-Yongle Sear Crate ineendlela ezahlukeneyo zokuhlangabezana neemfuno zenkcubeko ezahlukeneyo.

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