Yintoni ekufuneka ithathelwe ingqalelo xa usebenzisa izitya ze-petri?

Isitya se-perit sisitya selebhu esisetyenziselwa ukukhulisa i-microorganisms okanye inkcubeko yeseli. Ine-face i-disc-cerched isiseko se-disc kunye nesiciko. Izinto ze-petri dish zihlulwe zangamacandelo amabini, ikakhulu zeplastiki kunye neglasi. Izixhobo zezityalo, inkcubeko yentsholongwane, kunye neenkcubeko eziqhotyoshelweyo zeglasi zeeseli zezilwanyana zisetyenziswa rhoqo. Iplastiki yenziwa nge-polythylene kwaye ilungele i-inocraratrial inoculation, i-inoculation kunye nokuzahlula intsholongwane kunye nezixhobo zezityalo.

It was originally designed in 1887 by the bacteriologist Julius Richard Petri (1852-1921) under the German biologist Robert Koch, so it is also known as the "Godfather" . Isitya se-li petring "

1. Ukuhlamba izitya ze-perit

a) Ukuhamba: Iglasi entsha okanye esetyenzisiweyo kufuneka ifakwe emanzini kuqala ukuthambisa kwaye ichithe izinamathiselo. Iglasi entsha kufuneka ikhutshiwe ngamanzi empompo ngaphambi kokusetyenziswa, emva koko ifakwe kwi-5% ye-hydrochloric acid ubusuku bonke ngobusuku bonke; Iglasi esetyenzisiweyo idla ngokuhlala ineeprotein kunye neoyile eqhotyoshelwe kuyo, ekulula ukuhlamba emva komile. Ke ngoko, kufuneka ifakwe emanzini acocekileyo kwaye ikhutshiwe ngokukhawuleza emva kokusetyenziswa.

b) Ukuchitha: Beka iglasi enyangwe kwiglasi yokuhlambela amanzi, kwaye iqhekeza ephindaphindiweyo ngebrashi ethambileyo. Shiyanga indawo efileyo kwaye uphephe ukonakalisa ukugqiba komgangatho wesixhobo. Hlamba kwaye womisa indebe ecocekileyo yokukhanyisa.

c) Ukukhetha: Ukuzikhethela kukuthoba ezi zinto zichazwe apha ngasentla kwisisombululo sokucoca, okwaziwa njengesisombululo se-ACID, ukususa izinto ezinokuhlala kumphezulu we-oxid software. Ukuthathwa akufuneki kube kungabikho ngaphantsi kweeyure ezintandathu, ihlala ibusuku okanye ixesha elide. Zilumkele izixhobo.

d) I-HARINS: I-Tebleware kufuneka isuswe ngokupheleleyo ngamanzi emva kokukrazula kunye nokugabha. Ingaba izithambisi zicocwe emva kokufunyanwa okuchaphazela impumelelo okanye ukusilela kwenkcubeko yeselfowuni. Iimpahla zokuhlamba izandla. I-tenil nganye kufuneka iphindwe kabini "ikhutshiwe ngamanzi" ubuncinci amaxesha ali-14, kwaye ekugqibeleni ihlanjwe ngamaxesha ama-2-3 amanzi ngamanzi aphindwe kabini, omileyo okanye omileyo kwaye ipakishwe emva kokusetyenziswa okulandelayo.

e) I-Sterilization: Izitya ezilahlekileyo zeplastiki zeplastiki zihlala zibhangiswe yi-Irradiation okanye intambo yekhemikhali xa ishiya umzi-mveliso.

2. Ukwahlulahlula izitya ze-petri

a) Ngokwendlela yokusetyenziswa kwe-perit yesifungo, inokohlulwa ibe sisitya senkcubeko kunye nesitya se-bact.

b) Ngokwezixhobo ezahlukeneyo zokuvelisa, zahlulwe zaba zizitya zeplastiki zeplastiki kunye nezitya ze-petri zeglasi, kodwa zombini izitya ezingeniswayo ezingeniswayo ezingeniswayo, kodwa izitya ze-petri ezingenisayo kunye nezitya ezingekhoyo ze-petri kunye ne-petri petri.

c) Ngokutsho kobukhulu obahlukeneyo, ikwadla ngokwahlulwa-hlulwa kwi-petri izitya ezinemigangatho ye-35mm, i-60mm, 90mm kunye ne-150mm.

d) Ngokwesahlulelo esahlukileyo, oko kunokwahlulwa kuba zizitya ezi-2 ezixhotyisiweyo ezi-2, zahlula-hlulo zePetritive, njl.

e) Isitya se-perit sihlulwe saba ziindidi ezimbini, ikakhulu iplastiki kunye neglasi, kunye neglasi zinokusetyenziselwa izixhobo zokutyala, inkcubeko yentsholongwane kunye nenkcubeko yokubambelela kwiselfowuni. Iplastiki inokwenziwa nge-polyethylene, kwaye kukho izinto ezilahlekileyo kwaye zisebenzisa izinto ezininzi, ezilungele i-inoraltory inoculation, zokuyila, kunye nokuzityalwa kweebhaktiriya, kwaye zinokusetyenziselwa ukulinywa kwezixhobo zezityalo.

3. Imicimbi efuna ukuhoywa ekusebenziseni izitya zenkcubeko

a) Emva kokucoca kunye nokubulala iintsholongwane ngaphambi kokusetyenziswa, nokuba isitya se-petri sicocekile okanye asinampembelelo enkulu emsebenzini, esinokuchaphazela i-PH yePh. Ukuba kukho iikhemikhali ezithile, ziya kuthintela ukukhula kweentsholongwane.

b) Izitya ezisandula ukuthembeka kufuneka zitshiswe ngamanzi ashushu kuqala, emva koko zityhutywe kwisisombululo se-hydrochloric ngenxalenye ye-1% okanye i-2% yeeyure ezininzi zokususa amanzi asimahla, emva koko agxothiwe kabini ngamanzi amaninzi.

c) Ukuba ufuna ukuhlakulela i-bacteria, sebenzisa i-Steam-the Steam Steam (ngokubanzi 6.8 * 10 ukuya kwi-5 ye-10 ye-Steam Steam Steam), i-Sterilize kwi-120 ° C ukuba ibe ngamaqondo obushushu egumbi, okanye i-sterilize ngobushushu owomileyo , Oko kukuthi, faka isitya se-perit siyibeka kwi-oveni kwaye sigcine ubushushu malunga ne-120 ° C kwiiyure ezi-2 zokubulala iiseli zentsholongwane.

d) Izitya ze-petrilt kuphela zinokusetyenziselwa i-inoculation nenkcubeko.