Ukuphathwa kwezitya zenkcubeko linyathelo elibaluleke kakhulu ukuqinisekisa iziphumo ezichanekileyo, ezinokuthenjwa kunye neyokuphilayo ngexesha lenkcubeko yeselfowuni. Eli nqaku liza kuyichaza injongo, indlela yokusebenza, kunye neendlela ezilungileyo zokulinganisa i-TC kwaye zixoxe ngokubaluleka kwayo kuphando lwenkcubeko. 1. Injongo yokuqhubela phambili kwe-TC Unyango lwe-TC (okwaziwa ngokuba nonyango lwenkcubeko yezicubu) luphawu olukhethekileyo lwenkcubeko yezitya zokuphucula indawo yokuhamba, ukuthintela ukunamathela okubi okanye ukwandisa ukufakwa kweseli. Unyango lwe-TC lunokwenza ukuba iiseli zincamathisela ngcono ezantsi kwenkcubeko yesitya, kwaye inokukhuthaza ukukhula kweselokhrasi kunye necandelo, ngelixa kunciphisile umngcipheko wokufa kweseli ngenxa yokutshutshiswa kweseli ngenxa yokutshutshiswa kweseli. 2. Indlela yokulungisa i-TC Kukho iindlela ezahlukeneyo zokufumana unyango lwe-TC, kubandakanya neendlela zomzimba kunye neekhemikhali. Phakathi kwabo, indlela yokwenyama kukunciphisa uxinzelelo lomphezulu wenkcubeko ngesitya sokuncitshiswa komgangatho wokupheliswa kwenkunkuma yokuphakamisa intsholongwane; Eyona ndlela yekhemikhali kukusebenzisa izixhobo ezinje nge-siloxane ukurhweba umphezulu weSitya senkcubeko ukuphucula ukutshintsha komhlaba. 3. Intshukumo efanelekileyo yokuqhubela phambili kwe-TC Xa usenza unyango lwe-TC, la manqaku alandelayo kufuneka aqatshelwe: 3.1 Ukucocwa: Ngaphambi kokuqokelela isitya se-petri ukuya kunyango lwe-TC, kufuneka kuqinisekiswe ukuba umphezulu wayo uhlambulukile, onqinileyo nowomileyo. Oku kunokufezekiswa ngokusebenzisa i-sorganic ye-organic (njenge-ethanol okanye i-methanol) kunye nesibane se-UV. Ixesha lexesha: Ixesha lokuqhuba le-TC kufuneka lilawulwe ngokuchanekileyo. Izinto ezahlukileyo ze-dish kunye neentlobo zonyango zinokufuna amaxesha onyango. Ke ngoko, ngaphambi kokwenza unyango lwe-TC, kuya kufuneka ubhekise kwimiyalelo okanye uncwadi olunikezwe ngumthengisi, kwaye wenze uhlengahlengiso olufanelekileyo ngokweemfuno zovavanyo. 3.3 Ulawulo loMgangatho: Iziphumo zonyango lwe-TC ziya kuchaphazela iziphumo ezilandelayo zenkcubeko. Ukuqinisekisa ukuba i-batch nganye ye-perit inokufumana isiphumo sonyango olungaguqukiyo lwe-TC, uvavanyo lokuphindwayo kunye nolawulo lomgangatho lunokwenziwa ukuqinisekisa ukungqinelana kwe-batch nganye ye-Petri. 4. Ukubaluleka konyango lwe-TC lophando lwenkcubeko Unyango lwe-TC aluphuculi nje ngokufana kweseli encamathiselwe iselfowuni kunye nenqanaba lokukhula, kodwa inciphise ukufa kweseli nokukhuthaza ukwahlulahlulwa kweseli. Ezi nzuzo zinokudlala indima ebalulekileyo kwiinyanga ezininzi zenkcubeko, njengokuphuculwa kweseli, ukuhlolwa kweziyobisi, kunye novavanyo lwe-cytotototy, njl.
