Ukucoca isitya seNkcubeko xa usebenzisa

Isitya se-selt (i-150mm): Uyilo olukhethekileyo lokuphucula ukwandisa amandla esivalo kunye nezantsi kwesitya, iphucula ukungcola ngaxeshanye, inyuse i-adsen yodonga lwangaphakathi lwesitya senkcubeko.

Ukucoca amanyathelo kwiSusts zeNkcubeko yeSoftware:

Ngokubanzi, ihamba ngamanyathelo amane okuthoba, ukuqubha, ukuthathwa, kunye nokucoca.

cell culture dish

1. Ukuntywila: Iglasi entsha okanye esetyenzisiweyo kufuneka ifakwe emanzini kuqala ukuthambisa kwaye ichithe izinamathiselo. Iglasi entsha kufuneka ikhutshiwe ngamanzi empompo ngaphambi kokusetyenziswa, emva koko ifakwe ubusuku kwi-5% ye-hydrochloric acid; Iglasi esetyenzisiweyo ihlala ineeprotein ezininzi kunye neoyile eqhotyoshelwe kuyo, ekungekho lula ukuhlamba emva komomo, ke kufuneka ingene emanzini acocekileyo kwangoko emva kokusetyenziswa.

2. Ukuchitha: Beka iGradeyare enobuqili kwi-orgent amanzi kwaye iqhekeza ephindaphindiweyo ngebrashi ethambileyo. Musa ukushiya indawo efileyo kwaye uthintele umonakalo kumphezulu wokugqiba kwezixhobo. Hlamba kwaye womisa indebe ecocekileyo yokukhanyisa.

3. Ukuthathwa: Ukukhetha: Ukucofa kukuthoba izisombululo ezichazwe apha ngasentla kwisisombululo sokucoca, esaziwa njengesisombululo se-Acid, ukususa izinto ezihamba-jikelele kumphezulu we-oxillation ngesisombululo esiqinileyo sesisombululo esiqinileyo sesisombululo se-asidi. Ukuthathwa akufuneki kube kungabi ngaphantsi kweeyure ezintandathu, ihlala ibusuku okanye ixesha elide. Zilumkele izixhobo.

4. Hlambulula: Izitoni emva kokuqubha kunye nokuthathwa kufuneka kupheliswe ngamanzi. Nokuba izinxibo zicociwe emva kokuzithandela kuya kuyichaphazela ngokuthe ngqo impumelelo okanye ukusilela kwenkcubeko yeselfowuni. Ukuhlambela ngesandla