Ukutywina kunye nokungcolisa ungcoliseko kwiipleyiti ze-Multiwell

Ukulayisha kunye nokuphatha iiplate zenkcubeko ezininzi:

Iphepha lenkcubeko leseli lilandela umgaqo-siseko wokungqongqo kwe-asepsis engqongqo xa usenza imisebenzi yeseli. Yonke imisebenzi kufuneka yenziwe ngomgangatho nezenzululwazi, kwaye ayizukubangela ifuthe elongeziweyo ekukhuleni kweseli. Eyona ngxaki ixhaphakileyo yindlela yokuqinisekisa ukufana kweeseli emva kokudibanisa isampulu kwaye inciphise ifuthe lokutshintsha iphakathi kwiSiseko seSaba.

Buza:
Izithintelo ezingama-96 kunye ne-24 okanye izitya zenkcubeko ezihamba kakuhle, ezilungele umoya wezahlulo, kodwa ziya kutsala iibhakteria, ukubumba kunye nabanye abangcolisekileyo kukutyibilika?

Impendulo:
Isivalo sikhululekile kakhulu, esiya kwinkcubeko evulekileyo, kwaye injongo yoku kukuba se-ventilate (enyanisweni, kukwenza i-CO2 ngaphandle kweSitya senkcubeko kwaye sigcine ixabiso le-PH kwaye ligcine ixabiso lenkcubeko iphakathi)
Yonke into inezibonelelo kunye nezinto ezingalunganga, ezizonyukayo zonyusa ukubakho kongcoliseko. Ukongeza, oku kubangela ukuba ulwelo kwisitya ukuphelisa, okuphawulezileyo ukuze kwenziwe i-dosity yeziyobisi. Ke ngoko, kunyanzelekile ukuba la manyathelo mabini alandelayo ayimfuneko: a. Umoya kwi-incubator kufuneka icocekile (ukukhanya rhoqo, ukuthoba kotywala, kwaye i-inqubator kufuneka ivulwe kwaye ivalwe kancinci kangangoko) b. Umswakama kwi-incubator kufuneka igcinwe igcinwe kwi-100% (intsimbi enamanzi ashiyiweyo eshiyiweyo ebekwe kwi-incubator).
Njengesitya sepetri, ikwasisikhongozeli ngesivalo esinephantsi, kwaye ayiyi kungcoliswa. Ngakumbi ngenxa yoxinzelelo olubi lomoya oveliswe ngu "L" wekhava, kukho imicroorganisms eqhotyoshelwe eluthulini, kwaye uthuli oluqhutywa ngumbane lungakwazi ukudlula kwikhava lemveliso. Iziphumo zomoya kuphela ngokusahluka komoya, kwaye akukho moya moya uya kwenziwa, kuya kuya kuphela kwaye ungangeni nje intsholongwane.

Buza:
Sebenzisa ipleyiti ezingama-24, kukho imisebenzi kwezinye iiqula (kwibhentshi ye-Ultra-Count), kwaye kukho iiseli eziya kutyalwa kwezinye iqula. Ndikhathazekile ukuba oku kuya kubangela ungcoliseko. Andazi ukuba mandinikele ingqalelo?

Impendulo:
Ukuba umsebenzi usemgangathweni kwibhentshi ye-Ultra-Conch, kufanele ukuba ilungile. Ndicinga ukuba ungayisebenzisa ngokupheleleyo isiciko seplate yenkcubeko, zama ukubhengeza imingxunya ukuba isebenze, kwaye igubungele eminye imingxunya enezikhuselo.
Cinga ngokuqonda kwangaphambili ngaphambi kokuba usebenzise kwaye usebenzise yonke imingxunya; Ukuba ufuna ukusebenzisa imingxunya embalwa, unokusebenzisa elinye icala, kwaye ugubungele ukuphumla ngekhava. Ndiqhele ukusebenzisa umngxunya ofanelekileyo kuqala (kufanelekile ukongeza iisampulu ngasekunene).

Xa usebenza, sebenzisa izilayidi ezimbalwa zeglasi ukuphakamisa icala elinye leBhodi, musa ukuvula ikhava ngokupheleleyo, ngokubanzi akukho ngxaki.
Ukuhanjiswa kweseli engalinganiyo kunye nezisombululo

Buza:
Iiseli zinyuswe kwiplate yenkcubeko, kwaye iiseli zihlala zihlanganisa kwinxalenye ye-pepheral, indlela yokujongana nayo?

Impendulo:
Ndingabuza ukuba iiseli zakho zixutywe njani? Ngaba i-pipetting okanye ishukumisa iplate yenkcubeko? Ukuba yile yokugqibela, kwaye ukuba iyashukunyiswa kwisangqa, kusenokwenzeka ukuba iiseli ziya kuphoswa kwilungu le-peripheral ngenxa ye-centrifugal, ezikhokelela kwiiseli ezimbalwa phakathi. Ngaphezulu kweeveki ezine!
Nantsi indlela elungileyo: Ngaphambi kokuhlakulela iplate yembewu, ibeke iplate yenkcubeko kwi-incubator iiyure ezimbalwa zokugcwala emva koko uyikhuphe. Amandla kufuneka ukuba akhanye xa ukutyala iiseli. Ukongeza kancinci kuvumela ukumiswa kweseli ukuba kuhambelane nequla leplate, kwaye iiseli ezikhulisiweyo zikhula ngokulinganayo. Khumbula ukungaze uquze nge-shaker, okanye iiseli zakho ziya kudityaniswa kunye ngathi uthethile.
Incinci ububanzi be-hole yeplate yenkcubeko, ngokucacileyo le nto injalo. Le phenonon ayinakuphepheka ukuba ibe ziipleyiti ezingama-24 ukuya kwengama-96, kuba ulwelo lunamathela eludongeni ukuze isisombululo senkcubeko esequleni alwenzi inqanaba lolwelo, kodwa i-periphery iphezulu, njengesipili se-concave. Nangona kunjalo, xa usebenzisa ezi ntlobo zimbini zeeplate ze-orifice, akunakwenzeka ukongeza isisombululo senkcubeko ngenxa yemfuno yokungenelela, ukuze iiseli zibonakale "kunye nesisombululo senkcubeko. Kuxhomekeka ekubeni zithini izikhombisi. Ukuba yi-MTT, i-uRunokaiststochemistry iya kuchaphazela iziphumo ngenxa yokutshutshiswa kweseli.
Xa quka iiseli, unikele ingqalelo kumbhobho ngokulinganayo ukuthintela ukungcola, kunye nenani lesisombululo senkcubeko equleni kufuneka sanele. Ngokubanzi, yongeza isisombululo senkcubeko eyaneleyo xa usenza i-iculac, kwaye utshintsha isisombululo kube kanye xa ukongeza ungenelelo. Isixa seSisombululo seNkcubeko esisongelwe kwisisombululo sokungenelela silingana nesixa sezisombululo senkcubeko ngexesha le-inoculation ngexesha le-inoculation ngexesha le "iseti yomphetho" iya kuphuculwa.

Buza:
Into endayenzayo yayiluvavanyo lwe-plaque. Kungcono ukusasaza iiseli kwi-sub efanayo. Uninzi lweemingxunya zilungile xa intsholongwane ikhutshiwe, kwaye ezinye zazo azifani. Kukho umahluko omkhulu kwiqela leseli. Ndingathanda ukukubuza ukuba yeyiphi indlela oyisebenzisayo xa usongeza iiseli?

Impendulo:
Kubalulekile ekutyibilikeni iiseli ukuba zinqunyanyiswe ngaseseni emva kokuganga! Qiniseka ukuba inani leeseli kwiqumrhu ngalinye liyahambelana xa liqhekeza iplate!
Ewe, kukho izinto zokulungisa izinto. Ukufana phakathi kwequla kubaluleke kakhulu. Umzekelo, xa uyongeza amayeza, xuba iyeza emva kobuqhetseba ukuqinisekisa ukuba uxinzelelo lwequla ngalinye liyahambelana!
Ngaphaya koko, xa uyongeza iiseli kunye neziyobisi, iqela lovavanyo, iQela loLawulo kufuneka longezwe longezwe ukuze kuthintelwe umahluko kwisuti yokuxinanisa kunye noxinzelelo lweziyobisi olubangelwa zongeze iisampulu zongeze iisampulu zongezisanda!

Buza:
Ukuvuthuza sele iyunifomu, kodwa iplanga, imingxunya emi-6, kunye nemingxunya yama-24 ihlala ingalingani, kwaye igxile kancinci embindini. Ngapha koko, kuyacaca ukuba ukubala kwenziwa, kwaye emva kosuku okanye ezimbini, ukuxinana komngxunya ngamnye okwahlukileyo, oya kuthi uchaphazele ukufunyanwa. Ngaba ikhona indlela elungileyo?

Impendulo:
Ukuba usebenzisa i-pasteur Pipette, musa ukuncanca kakhulu ixesha ngalinye, kuba iiseli zomphunga ziya kungcungcutheka ngokuzenzekelayo, ngokufuthi inani leemingxunya zokuqala likhulu, kwaye ulwelo luhlala lukhulu, kwaye ulwelo luya kuhlala lukhulu, kwaye ulwelo luhlala lukhulu Landela indlela ye-S-SM.
Ukuba usebenzisa i-pipette, iingcebiso kufuneka zenziwe kwaye zisuswe ukunqanda ukonakalisa iiseli, ukuze lonke ixesha lithathwe kwiqula. Ichanekile ngakumbi ukongeza umngxunya omnye-nye ngentloko. Kodwa nikela ingqalelo ekuxubeni ukunqunyanyiswa.

Ukuseta iqela elifanayo, kufuneka kube likhulu ngokwaneleyo (ungabala).

Sukugungqa emva kokubetha, njengokungcungcuthekisa kuya kubangela ukuba iiseli zidibanise kwiziko. Kungcono ukucwangcisa kube kanye.

I-poirous ye-pote yourlie yonyango ihlala ithathela ingqalelo ulawulo olusemgangathweni njengobomi beshishini, kwaye yalandela ukuphuculwa okuqhubekayo kokhuphiswano lweshishini. Inkampani ihlala inamathela kwiimoya zeshishini lokuthobela imithetho nemigaqo, ukuba ingqinelwa kukuziqeqesha, kwaye igcina intengiso yomgangatho kunye nenkonzo egqwesileyo, kwaye intlanganiso iimfuno zabathengi ukuya phambili. . Ukuphumelela ukuphumelela kunye nabathengi yinjongo yethu yophuhliso. Yongyie kwezonyango! Iqabane lakho elithembekileyo. Sizimisele ukusebenzisana ngokunyaniseka kunye nawe ukuze wenze ikamva elingcono.