Ibala lesikhokelo seQub

1. I-terum ye-terum i-tube ebomvu
Ityhubhu yokuqokelelwa kwegazi iqulethe akukho songezo, akukho zithako ze-anticoulrent okanye i-procoguent, kuphela vacuum. Isetyenziselwa i-biechemistry ye-biochemistry ye-serum, ibhanki yegazi kunye neemvavanyo ezinxulumene ne-serology, uvavanyo lwe-biochemical kunye novavanyo lwe-biochemical, ezinje nge-syphilis, i-hepatitis B Akudingiswanga, njl. Njl. Uhlobo lohlobo oluqingqiweyo yi-serum. Emva kokuba igazi litsaliwe, libekwe kwi-37 ° C yokuhlala amanzi ngaphezulu kwemizuzu engama-30, i-Sereriguged, kwaye i-serum ephezulu isetyenziselwa ukusetyenziswa kamva.
2. I-tuum yokuqokelelwa kwe-serum ekhawulezayo ye-orenji yegazi
Kukho i-gogulant kwi-tube yokuqokelelwa kwegazi ukukhawulezisa inkqubo ye-quage. Ityhubhu ye-serum ekhawulezileyo inokudibanisa igazi eliqokelelweyo kwimizuzu emi-5. Ilungele uvavanyo lokorim kaxakeka. Yeyona tyhubhu ixhaphakileyo yovavanyo lwe-biochemistry yemihla ngemihla, ubugomi, i-serum, i-hormones, njl njl. Xa iqondo lobushushu liphantsi, lingabekwa kwibhafu lamanzi angama-37 ° CAD nge-10 ukuya kwi-12min, kunye ne-serum ephezulu ayinakwenzeka ukuba isetyenziswe kamva.
3. I-cap yegolide ye-intert yokwahlula i-gub yegazi yeTub yeTub yeTub eTshayina
I-Intert yahlula ijeli kunye ne-cougulant yongezwa kwityhubhu yokuqokelelwa kwegazi. Iisampulu zizinzile iiyure ezingama-48 emva kokuqina kwenkululeko. I-procagunts inokuvula ngokukhawuleza indlela yokudibanisa kunye nokukhawulezisa inkqubo ye-RAGS. Uhlobo lohlobo olulungiselelwe yi-serum, elilungele uvavanyo lwe-bieurmical ye-biochemical kunye ne-pharmaconkinetictic. Emva kokuqokelela, ingenayo kwaye idibanise amaxesha angama-5-8, yima nkqo iminyaka engama-20 ukuya kwengama-20, kwaye i-Centrifuyimbo ibeka i-supernatant yokusetyenziswa kamva.
4. I-sodium citictrate esr tube ye-tube emnyama
Uxinzelelo lwesodium citirate olufunekayo kuvavanyo lwe-ESR yi-3.2% (ilingana ne-0.109 mol / L), kwaye umyinge we-anticouhlant ngegazi ngu-1: 4. Iqukethe i-0.4ml ye-3.8% ye-sodium ye-sodium, kwaye iyyy igazi kwi-2.0ml. Le yityhubhu yovavanyo olukhethekileyo kwi-Erythrocyte Sediment. Uhlobo lwesampulu yiplasma, elungele i-ESR. Ngokukhawuleza emva komzobo wegazi, ingenayo kwaye xuba amaxesha ama-5-8. Shake kakuhle ngaphambi kokusetyenziswa. Umahluko phakathi kwawo kunye netyhubhu yovavanyo kwi-caictor yezinto kukuba uxinzelelo lwe-anticoursula lwahlukile kumyinge wegazi, olungafanele ludideke.
5. I-sodium tict yovavanyo lwe-tube ye-tube ye-tube ye-tube eluhlaza okwesibhakabhaka
I-sodium citict ikakhulu isebenza njenge-anticogolant ngokuthintela i-calcium ye-calcium kwiisampulu zegazi. Uxinzelelo lwe-anticourlant lucetyiswa yiKomiti yeSizwe yokuCwangciswa kweeLabhoratri zeklinikhi yi-3.2% okanye i-3.8% (ilingana ne-0.109l / L), kwaye i-0.129l / l), kwaye i-0.128mol / L) Ityhubhu yokuqokelelwa kwegazi iqulethe malunga ne-0.2 ml ye-3.2% yesodium ye-anticouhlalar, kwaye igazi liqokelelwa kwi-2.0 ml. Uhlobo lokulungiselela umzekelo ligazi liphela okanye iplasma. Ngokukhawuleza emva kokuqokelela, ingenayo kwaye xuba amaxesha ama-5-8. Emva kokuqina kweyekhulu, thatha iplasma ephezulu. Ilungele uvavanyo lokudibana, i-PT, i-PT, i-Aptt, uviwo lweFaCICT.
6. I-heparin anticojoiction tube ene-green cap
I-Heparin yongezwa kwityhubhu yokuqokelelwa kwegazi. Ngoku ngqo ifuthe le-antithrombin, enokuphinda iqhekeze ixesha lokudibana. Ngongxamiseko kwaye uninzi lweemvavanyo zebhayoloji, umsebenzi wezintso, iswekile yegazi, uvavanyo lwegazi, i-hematocrict sedication, kodwa hayi Ilungele uvavanyo lwegazi. I-Heparin egqithisileyo inokubangela ukwahlukaniswa kweeseli zegazi ezimhlophe kwaye azinakusetyenziselwa ukubala kweeseli ezimhlophe zegazi. Akukhethiwe kukwahlulwa kwe-leukiccyte kuba inokwenza ifilimu yegazi ibe nefuthe ngemvelaphi eluhlaza okwesibhakabhaka. Ingasetyenziselwa intengiso yegazi. Uhlobo lwesampulu yiplasma. Ngokukhawuleza emva kokuqokelela igazi, ingenayo kwaye xuba amaxesha ama-5-8, kwaye uthathe iplasma ephezulu yokusetyenziswa.
7. Isibane esiluhlaza esiluhlaza se-plasma yokwahlulahlula
Ukongeza i-heparin lithium ye-anticoursular kwi-intert ityhubhu ingafezekisa injongo yokwahlulahlula iplasma, elona tyelelo lilungileyo lovavanyo lwe-elektrolyte. Ngongxamiseko kwaye uninzi lweemvavanyo zebhayoloji, umsebenzi wezintso, iswekile yegazi, iswekile yegazi, njl. Njl. Ingasetyenziselwa intengiso yegazi. Uhlobo lwesampulu yiplasma. Ngokukhawuleza emva kokuqokelela igazi, ingenayo kwaye xuba amaxesha ama-5-8, kwaye uthathe iplasma ephezulu yokusetyenziswa.

8. I-potassium oxilate ye-oxpalailate / sodium fluoride igrey
Isodium fluoride sisidenge esibuthathaka, esihlala sisetyenziswa ngokudibeneyo ne-potassium oxilate okanye i-sodium ye-sodium ye-oxmaride i-slodium ye-oxpalalate. I-4MG yalo mxube unokwenza i-1ml yegazi ingaphakathi kweentsuku ezingama-23 kwaye ithintele ukubola kweswekile. Ayinakusetyenziselwa ukumiselwa kwe-Urea ngeNdlela ye-Urease, okanye ukumiselwa kwe-alkaline phosphetase kunye ne-amssebese. Kucetyiswa ukuba kuvavanywe iswekile yegazi. Ine-sodium fluoride okanye i-potassium oxilate okanye i-shukudium ye-Ethylediaminetetracetracetracetrace (E-EdTA-NA) i-Spray, enokuthi ikhuphe imisebenzi ye-glucose metabolism. Emva kokuzoba igazi, ingena kwaye udibanise amaxesha ama-5-8. I-Plasma ye-Liqual igcinelwe ukusetyenziswa, kwaye yityhubhu ekhethekileyo yomlinganiselo weglucose ekhawulezayo.
9. I-EDTA Anticooag
I-Ethyyediaminetetratetic acid (Edta, ubunzima bemolekyuli 292) kwaye iityuwa zayo yi-Amino Polycarboxylic Acid, kwaye zizinto ezikhethiweyo zovavanyo lwe-Amino, kwaye zivavanywa i-hemoglobin, kunye neemvavanyo zeqela. Ayilungelanga uvavanyo lomsebenzi wovavanyo kunye novavanyo lomsebenzi we-caltelet, okanye ukumiselwa kwe-calcium ion, i-potassium ion, i-sodium ye-ion, i-iron ye-ion, i-leucine aminopeptifase, ilungele uvavanyo lwe-PCR. SPRAY I-100ML ye-2.7% ye-EDTA-K2 kudonga lwangaphakathi lwetyhubhu, ukuvuthuza owomileyo ngamaxesha angama-2ml, kwaye umxube kakuhle ukuze usetyenziswe. Uhlobo lwesampulu igazi liphela, elifuna ukuba lidityaniswe nezodwa ngaphambi kokusetyenziswa.